Vial Guide

Thymosin Alpha-1 5 mg vial

How much bacteriostatic water to add to a 5 mg vial of Thymosin Alpha-1, the syringe units for each common dose, and how many doses the vial holds.

Water
1 mL
Strength
5 mg/mL
Common dose
1.6 mg
Draw
32 units0.32 mL
Doses per vial
3

The short answer

Mixed with 1 mL of bacteriostatic water, a 5 mg vial of Thymosin Alpha-1 holds 5 mg/mL. A 1.6 mg dose is then 32 units (0.32 mL) on a U-100 insulin syringe, and the vial holds about 3 doses of that size. Taken 2× per week, it lasts about 11 days.

Approved drug. Approved outside the US as Zadaxin: for chronic hepatitis B in countries such as Indonesia (and in some, hepatitis C), and in Italy only as an add-on to flu vaccination in people with weakened immune systems. Not FDA-approved.
Thymosin Alpha-1 page

How much water

The water only sets the strength; the dose and the number of doses in the vial stay the same. Draws are on a U-100 insulin syringe, where 1 unit is 0.01 mL.

Water addedStrengthPer syringe unitDraw for 1.6 mg
1 mL5 mg/mL50 mcg32 units (0.32 mL)
2 mL2.5 mg/mL25 mcg64 units (0.64 mL)
3 mL1.667 mg/mL16.7 mcg96 units (0.96 mL)

Dose chart

Syringe units for each common dose at each water volume. Select a cell to open it in the calculator.

DoseWater added
1 mL2 mL3 mL
1.6 mg

Draws over 100 units show in mL. Faded values are under 2 units, which is hard to measure. Check that your vial holds the larger volumes.

Where the doses come from

  • Label 1.6 mg under the skin twice weekly, 3–4 days apart. For hepatitis B the course is 6 months (52 doses). Some countries also approve it with interferon for hepatitis C.

Doses are what labels, trials or community reports reference, not recommendations. Thymosin Alpha-1’s page has the cycle, side effects, cautions and sources.

Mixing and storage

  1. PrepareLet a refrigerated vial come to room temperature, and wipe both stoppers with an alcohol swab.
  2. Add 1 mLDraw 1 mL of bacteriostatic water and let it run slowly down the inside wall of the vial.
  3. Swirl, don’t shakeSwirl gently until the powder dissolves and the liquid is clear.
  4. Label itWrite the date and the strength, 5 mg/mL, on the vial.

Storage: Label: 2–8 °C. Mixed: 2–8 °C.

The reconstitution guide covers each step, and the units page explains syringe markings.

Questions

How much water do I add to a 5 mg vial of thymosin alpha-1?

There’s no single right amount: the water only sets the strength. With 1 mL of bacteriostatic water, a 5 mg vial holds 5 mg/mL. With 2 mL it’s 2.5 mg/mL; with 3 mL it’s 1.667 mg/mL. Less liquid makes each dose a smaller draw; more makes small doses easier to measure.

How many units is 1.6 mg of thymosin alpha-1?

With 1 mL in a 5 mg vial, 1.6 mg is 32 units (0.32 mL) on a U-100 insulin syringe. With 2 mL, 64 units (0.64 mL); with 3 mL, 96 units (0.96 mL).

How many doses are in a 5 mg vial of thymosin alpha-1?

About 3 doses of 1.6 mg. Taken 2× per week at 1.6 mg, it lasts about 11 days.

How long does a mixed vial of thymosin alpha-1 keep?

Label: 2–8 °C. Mixed: 2–8 °C.

Other vial sizes

Sources

  • Zadaxin (thymalfasin) drug information (RxList)
  • FDA advisory committee briefing: thymosin alpha-1 (2024)

The math on this page uses the doses and storage on Thymosin Alpha-1’s page.