Vial Guide

NAD+ 500 mg vial

How much bacteriostatic water to add to a 500 mg vial of NAD+, the syringe units for each common dose, and how many doses the vial holds.

Water
3 mL
Strength
166.7 mg/mL
Common dose
50 mg
Draw
30 units0.30 mL
Doses per vial
10

The short answer

Mixed with 3 mL of bacteriostatic water, a 500 mg vial of NAD+ holds 166.7 mg/mL. A 50 mg dose is then 30 units (0.30 mL) on a U-100 insulin syringe, and the vial holds about 10 doses of that size. Taken 3× per week, it lasts about 3.3 weeks.

Human studies. Human data are thin: one small IV infusion study (8 men given NAD+, 2019) and a 2026 industry-funded preprint that tested 100 mg by IV, IM and SC injection for 3 days. A 2026 systematic review found no outcome trials of injected NAD+. Not an approved drug.
NAD+ page

How much water

The water only sets the strength; the dose and the number of doses in the vial stay the same. Draws are on a U-100 insulin syringe, where 1 unit is 0.01 mL.

Water addedStrengthPer syringe unitDraw for 50 mg
1 mL500 mg/mL5 mg10 units (0.10 mL)
2 mL250 mg/mL2.5 mg20 units (0.20 mL)
3 mL166.7 mg/mL1.67 mg30 units (0.30 mL)

Dose chart

Syringe units for each common dose at each water volume. Select a cell to open it in the calculator.

DoseWater added
1 mL2 mL3 mL
50 mg
60 mg
75 mg
100 mg
150 mg
200 mg

Draws over 100 units show in mL. Faded values are under 2 units, which is hard to measure. Check that your vial holds the larger volumes.

Where the doses come from

  • Community Injection: 50–100 mg, 2–3× per week. Some load daily for 7–10 days first.
  • Community IV (clinic): 250–500 mg per session, infused slowly over 2–4 hours.

Doses are what labels, trials or community reports reference, not recommendations. NAD+’s page has the cycle, side effects, cautions and sources.

Mixing and storage

  1. PrepareLet a refrigerated vial come to room temperature, and wipe both stoppers with an alcohol swab.
  2. Add 3 mLDraw 3 mL of bacteriostatic water and let it run slowly down the inside wall of the vial.
  3. Swirl, don’t shakeSwirl gently until the powder dissolves and the liquid is clear.
  4. Label itWrite the date and the strength, 166.7 mg/mL, on the vial.

Storage: Powder: 2–8 °C or colder. Mixed: 2–8 °C; guides cite 2–4 weeks.

The reconstitution guide covers each step, and the units page explains syringe markings.

Questions

How much water do I add to a 500 mg vial of NAD+?

There’s no single right amount: the water only sets the strength. With 3 mL of bacteriostatic water, a 500 mg vial holds 166.7 mg/mL. With 1 mL it’s 500 mg/mL; with 2 mL it’s 250 mg/mL. Less liquid makes each dose a smaller draw; more makes small doses easier to measure.

How many units is 50 mg of NAD+?

With 3 mL in a 500 mg vial, 50 mg is 30 units (0.30 mL) on a U-100 insulin syringe. With 1 mL, 10 units (0.10 mL); with 2 mL, 20 units (0.20 mL).

How many doses are in a 500 mg vial of NAD+?

About 10 doses of 50 mg, or 3 of 150 mg or 2 of 200 mg. Taken 3× per week at 50 mg, it lasts about 3.3 weeks.

How long does a mixed vial of NAD+ keep?

Powder: 2–8 °C or colder. Mixed: 2–8 °C; guides cite 2–4 weeks.

Other vial sizes

Sources

  • Grant et al., NAD+ metabolome during a 6-hour IV infusion (Front Aging Neurosci 2019) doi:10.3389/fnagi.2019.00257
  • Gallagher et al., NAD+ supplementation systematic review (Ageing Res Rev 2026) PMID 41655607

The math on this page uses the doses and storage on NAD+’s page.

By Vial Guide editorsLast reviewed

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