AOD9604 5 mg vial
How much bacteriostatic water to add to a 5 mg vial of AOD9604, the syringe units for each common dose, and how many doses the vial holds.
- Water
- 2 mL
- Strength
- 2.5 mg/mL
- Common dose
- 300 mcg
- Draw
- 12 units0.12 mL
- Doses per vial
- 16
The short answer
Mixed with 2 mL of bacteriostatic water, a 5 mg vial of AOD9604 holds 2.5 mg/mL. A 300 mcg dose is then 12 units (0.12 mL) on a U-100 insulin syringe, and the vial holds about 16 doses of that size. Taken 1× per day, it lasts about 2.4 weeks.
How much water
The water only sets the strength; the dose and the number of doses in the vial stay the same. Draws are on a U-100 insulin syringe, where 1 unit is 0.01 mL.
| Water added | Strength | Per syringe unit | Draw for 300 mcg |
|---|---|---|---|
| 1 mL | 5 mg/mL | 50 mcg | 6 units (0.06 mL) |
| 2 mL | 2.5 mg/mL | 25 mcg | 12 units (0.12 mL) |
| 3 mL | 1.667 mg/mL | 16.7 mcg | 18 units (0.18 mL) |
Dose chart
Syringe units for each common dose at each water volume. Select a cell to open it in the calculator.
| Dose | Water added | ||
|---|---|---|---|
| 1 mL | 2 mL | 3 mL | |
| 250 mcg | |||
| 300 mcg | |||
| 400 mcg | |||
| 500 mcg | |||
Draws over 100 units show in mL. Faded values are under 2 units, which is hard to measure. Check that your vial holds the larger volumes.
Where the doses come from
- Community 250–500 mcg once daily, most often 300 mcg.
Doses are what labels, trials or community reports reference, not recommendations. AOD9604’s page has the cycle, side effects, cautions and sources.
Mixing and storage
- PrepareLet a refrigerated vial come to room temperature, and wipe both stoppers with an alcohol swab.
- Add 2 mLDraw 2 mL of bacteriostatic water and let it run slowly down the inside wall of the vial.
- Swirl, don’t shakeSwirl gently until the powder dissolves and the liquid is clear.
- Label itWrite the date and the strength, 2.5 mg/mL, on the vial.
Storage: Powder cold and dark. Mixed: 2–8 °C; most guides use it within 3–6 weeks.
The reconstitution guide covers each step, and the units page explains syringe markings.
Questions
How much water do I add to a 5 mg vial of AOD9604?
There’s no single right amount: the water only sets the strength. With 2 mL of bacteriostatic water, a 5 mg vial holds 2.5 mg/mL. With 1 mL it’s 5 mg/mL; with 3 mL it’s 1.667 mg/mL. Less liquid makes each dose a smaller draw; more makes small doses easier to measure.
How many units is 300 mcg of AOD9604?
With 2 mL in a 5 mg vial, 300 mcg is 12 units (0.12 mL) on a U-100 insulin syringe. With 1 mL, 6 units (0.06 mL); with 3 mL, 18 units (0.18 mL).
How many doses are in a 5 mg vial of AOD9604?
About 16 doses of 300 mcg, or 12 of 400 mcg or 10 of 500 mcg. Taken 1× per day at 300 mcg, it lasts about 2.4 weeks.
How long does a mixed vial of AOD9604 keep?
Powder cold and dark. Mixed: 2–8 °C; most guides use it within 3–6 weeks.
Other vial sizes
Sources
- Stier et al., safety and tolerability of AOD9604 in humans (J Endocrinol Metab 2013) doi:10.4021/jem157w
- FDA advisory committee briefing: AOD-9604 (December 2024)
The math on this page uses the doses and storage on AOD9604’s page.